SMIBIOTICS Apollo BS

SMIBIOTICS Apollo BS is a unique patented strain of Bacillus subtilis XDY-1105.SMIBIOTICS Apollo BS produces a variety of antibacterial metabolites to inhibit pathogenic Clostridium perfringens and other Clostridium, reduce the growth of aerobic harmful microorganisms, and maintain intestinal health effectively. It can consume intestinal oxygen and produce good amount of digestive enzymes effectively in animal digestive tract.

Active component:

Bacillus subtilis XDY-1105

Features:

1. Patented strain (patented No.:201310616703.2).
2. Stable under normal commercial pelleting conditions and in storage of commercial feeds.
3. Excellent inhibitory ability against clostridium perfringens and other pathogenic bacteria.

Characteristics：

1. High spore uniformity, strong stress resistance

2. Outstanding antibacterial ability and compatible with other probiotics

Inhibitory toward various strains of pathogens

Zone of inhibition: sensitive-S <10mm - low sensitive; 10-14mm - middle sensitive; 15-20mm - high sensitive; >20mm - strong sensitive

No antagonism with beneficial microorganism

Note: The longitudinal strip : APOLLO BS, The horizontal strip is probiotics

3. Produce enzymes and has strong digestive ability

 

Benefits：

1. Reduce the survival of Clostridium enteritis and other bacterial diseases
Produce a variety of antibacterial metabolites, inhibit the growth of pathogenic microorganisms such as Clostridium perfringens, clostridium difficile, Staphylococcus aureus and Campylobacter jejuni.

2. Maintain intestinal flora balance
Inhibit the over reproduction of other harmful pathogenic microorganisms and regulate the balance of intestinal flora by means of biological oxygen capture, nutritional competition, and spatial competition.

3. Promote nutrient digestion
Produce good amount of digestive enzymes to assist digestion, improve nutrient utilization and decrease metabolic diarrhea .

4. Improving animal rearing environment
Reduce the concentration of harmful gases in the enclosure, improve the breeding environment, and reduce the occurrence of stress and respiratory diseases .

 

Application:

1. Prevention effect:

Table 1 Experimental animals: total 3200 25-day old broilers(Cobb500),800 chickens in each group.

Experimental time		A Apollo BS Low-dose prevention	B Apollo BS high-dose prevention	C Bacterial attack	D Normal
d1-3	Preventive treatment	l00g/t	300g/t	-	-
d4-8	Bacterial attack'	+	+	+	-
d9	Slaughter

Note: 1.Take Clostridium perfringens 1 * 10⁹ cfu l chicken / time in the morning and evening.twice a day
2.Rectal feces samples were taken from all experimental groups,and the total number of colonies was determined by dilution withthe same multiple.

Table 2.CFU of C.perfringens in chicken intestinal contents

Treatment	Cobb (CFU/mL)
A(Apollo BS Low-dose prevention)	4.93×106
B(Apollo BS high-dose prevention )	4.10×106
C(Bacterial attack)	2.20×108
D(normal)	≤103

Figure 1.Intestinal wall changes at day 9

Note: A1/A2: Low-dose cure; B1/B2: high-dose cure; C1/C2: Bacterial attack; D1/D2: Normal

Conclusion: After prevention with Apollo BS, it can significantly reduce the intestinal injury caused by C. perfringens.

2. Cure effect:

Table 3.Experimental animals: total 2000 25-day old broilers(Cobb500),400 chickens in each group.

Experimental time		A Apollo BS Low-dose prevention	B Apollo BS high-dose prevention	C Ampicillin cure	D Bacterial attack	E Normal
d1-5	Bacterial attack'	+	+	+	+	-
d6-8	Cure	l00g/t	300g/t	300g/t	-	-
d9	Slaughter

Note: 1. Take Clostridium perfringens 1 * 10⁹ cful chicken / time in the moming and evening. twice a day
2. Rectal feces samples were taken from allexperimental groups,and the total number of colonies was determined by dlution withthe same multiple.

Table 4.CFU of C.perfringens in chicken intestinal contents

Treatment	Cobb (CFU/mL)
A(Apollo BS Low-dose prevention)	1.75×107
B(Apollo BS high-dose prevention )	1.10×106
C(Ampicillin cure)	1.97×106
D(Bacterial attack)	5.00×108
E(Normal)	1.30×103

Figure 2.Intestinal wall changes at day 9

Note: A1/A2: Low-dose cure; B1/B2: high-dose cure; C1/C2: ampicillin treatment; D1/D2: Bacterial attack; E1/E2: Normal

Conclusion: After broilers were infected with Clostridium perfringens, Apollo BS treatment effectively reduced the intestinal damage, and its effect was similar to that of antibiotics.

 

Dosage:

Viable count : 1×10¹⁰ CFU/g

Poultry: 200g - 300g/ton complete feed

Piglets and sows: 200g– 300g/ ton complete feed

Growing and finishing pigs: 100g– 200g/ ton complete feed